Journal: Bio-protocol
Article Title: Selective Isolation of TOP3B•mRNA Covalent Intermediates Using Denaturing Oligo-dT Pulldown
doi: 10.21769/BioProtoc.5627
Figure Lengend Snippet: Onto the bottom piece of the slot blot apparatus, two pieces of filter paper should be placed only when using the Bio-Dot apparatus . For all slot blots, two pieces of positively charged nylon membrane, followed by 1 nitrocellulose membrane, should be placed before securing the top slot blot piece in place.
Article Snippet: If using the Bio-Rad slot blot apparatus, two pieces of filter paper (equilibrated in 20 mM Tris-HCl) should be added below the nylon and nitrocellulose membranes ( ).
Techniques: Dot Blot, Membrane